解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::In most cases, anti-protein aptamers are selected by systematic evolution of ligands by exponential enrichment (SELEX) using purified recombinant protein targets. Cell surface proteins, however, are not easy targets for SELEX due to the difficulties associated with their purification. Here, we developed a novel SELEX ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2006.02.004
更新日期:2006-07-01 00:00:00
abstract::To examine the role of the C-terminal domain in the chaperone function of trigger factor (TF), a number of truncation mutants were constructed, namely: TF419, TF389, TF380, TF360, TF344, and TF251, in which the C-terminal 13, 43, 52, 72, 88 residues or the entire C-domain were deleted, respectively. Co-expression of m...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2005.11.006
更新日期:2006-06-01 00:00:00
abstract::Potato virus A (PVA), a potyvirus with a (+)ssRNA genome translated to a large polyprotein, was engineered and used as a gene vector for expression of heterologous proteins in plants. Foreign genes including jellyfish GFP (Aequorea victoria) encoding the green fluorescent protein (GFP, 27 kDa) and the genes of human o...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2005.10.010
更新日期:2006-05-01 00:00:00
abstract::Apolipoprotein E (apoE) has been genetically linked to late-onset Alzheimer's disease. From the three common alleles (epsilon2, epsilon3 and epsilon4), epsilon4 has been suggested to promote amyloid beta (Ass) plaque fibrillation, one hallmark of Alzheimer's disease. It has been demonstrated that altered lipid content...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2005.10.007
更新日期:2006-05-01 00:00:00
abstract::The cartilage tissue has a limited self-regenerative capacity. Tissue-engineering represents a promising trend for cartilage repair. The present study was aimed to develop a biomaterial formulation by combining fragments of chitosan hydrogel with isolated rabbit or human chondrocytes. We first reported the properties ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2006.03.002
更新日期:2006-05-01 00:00:00
abstract::The Rap family of small GTP-binding proteins is composed by four different members: Rap1A, Rap1B, Rap2A and Rap2B. In this work we report the identification and characterization of a fifth member of this family of small GTPases. This new protein is highly homologous to Rap2A and Rap2B, binds labeled GTP on nitrocellul...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2005.08.007
更新日期:2006-03-01 00:00:00
abstract::A d-carbamoylase from Sinorhizobium morelens S-5 was purified and characterized. The enzyme was purified 189-fold to homogeneity with a yield of 19.1% by aqueous two-phase extraction and two steps of column chromatography. The enzyme is a homotetramer with a native molecular mass of 150 kDa and a subunit relative mole...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2005.05.013
更新日期:2006-03-01 00:00:00
abstract::Developing a logical and rational methodology for obtaining vaccines, especially against the main parasite causing human malaria (P. falciparum), consists of blocking receptor-ligand interactions. Conserved peptides derived from proteins involved in invasion and having high red blood cell binding ability have thus bee...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2005.07.006
更新日期:2006-02-01 00:00:00
abstract::We identified a T-to-C mutation 2 nucleotides (nt) upstream from the AG in a GT-AG intron between exons 2 and 3 in the human T-cell leukemia virus type I (HTLV-I) tax mRNA. This mutation resulted in the preferential usage of an alternative splice site, causing a 75-nt elongation of tax mRNA and reduced production of v...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2005.07.013
更新日期:2006-02-01 00:00:00
abstract::Synthesis of the tripeptide Z-Phe-Arg-SerNH2 has been accomplished by a recombinant cysteine protease, cathepsin L1 from liver fluke (Fasciola hepatica), using Z-Phe-Arg-OMe as acyl acceptor and SerNH2 as nucleophile in 0.1 M ammonium acetate pH 9.0-12.5% v/v acetonitrile at 37 degrees C. LC-MS detection indicated tri...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2005.06.004
更新日期:2006-01-01 00:00:00
abstract::Herein, we report the purification and biochemical characterization of a novel bi-functional protein proteinase/amylase inhibitor from the dietary leguminous pulse Phaseolus aureus Roxb. (Vigna radiata L.) by means of acetic acid precipitation, salt fractionation, ion-exchange chromatography (DEAE-cellulose) and affin...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2005.05.007
更新日期:2005-12-01 00:00:00
abstract::The preadipocyte cell lines 3T3-L1 and 3T3-F442A are widely used to study the cellular mechanisms of preadipocyte differentiation and mature adipocyte functions. However, transfection with naked DNA is inefficient in these cell lines. Adenoviral gene transfer is a powerful technique to induce high levels of transgene ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2005.05.010
更新日期:2005-11-01 00:00:00
abstract::Most evolutionists agree to consider that our present RNA/DNA/protein world has originated from a simpler world in which RNA played both the role of catalyst and genetic material. Recent findings from structural studies and comparative genomics now allow to get a clearer picture of this transition. These data suggest ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2005.03.015
更新日期:2005-09-01 00:00:00
abstract::Oligonucleotides are multifunctional molecules which can interfere with gene expression by different mechanism such as antisense, RNA interference, ribozymes, etc. For most in vivo diagnostic and therapeutic applications, oligonucleotides need to be delivered to the intracellular compartment of a specific organ, a dif...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2005.04.013
更新日期:2005-09-01 00:00:00
abstract::In all living cells 3' ends of RNA are posttranscriptionally elongated or shortened by nucleotidyl transferases and ribonucleases. The detailed analysis of the rpsO mRNA of Escherichia coli presented here demonstrates that transcription terminates in vivo at two sites located seven and eight nucleotides downstream fro...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2005.02.012
更新日期:2005-09-01 00:00:00
abstract::Bacterial superantigens (SAg) are the most potent activators of human T lymphocytes and recombinant immunotoxin using bacterial SAg shows promising clinical values. To engineer superantigen for immunotherapy of hepatocellular carcinoma, we genetically fused the superantigen staphylococcus enterotoxin A (SEA(D(227)A)) ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2005.04.005
更新日期:2005-08-01 00:00:00
abstract::A major tolerance mechanism that functions to replicate damaged genomic DNA across lesions that have escaped elimination by repair mechanism is translesion DNA synthesis (TLS). DNA polymerase kappa (Pol kappa), a specialised low-fidelity DNA polymerase which is able to perform DNA synthesis across several damaged base...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2005.02.007
更新日期:2005-07-01 00:00:00
abstract::The considerable affinity of tissue transglutaminase for heparin was the basis for use of heparin-based affinity matrices for enzyme purification. Interaction of transglutaminase with heparin might mimic the physiological binding to membrane heparan sulfates, accounting for the limited but significant fraction of enzy...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2005.01.012
更新日期:2005-06-01 00:00:00
abstract::Phosphatidic acid phosphohydrolase-1 (PAP-1) activity is reversibly inhibited by fatty acids and their acyl-CoA esters and it appears paradoxical that these effectors have been reported to increase the liver's esterification capacity by translocating the rate-limiting enzyme PAP-1 from cytosol to the endoplasmic retic...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2005.01.003
更新日期:2005-05-01 00:00:00
abstract::Matrix metalloproteinases (MMPs) are a family of zinc endopeptidases that process growth factors, growth factor binding proteins, cell surface proteins, degrade extracellular matrix (ECM) components and thereby play a central role in tissue remodeling and tumor progression. Membrane-type matrix metalloproteinases (MT-...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2004.07.012
更新日期:2005-03-01 00:00:00
abstract::Enterocin CRL35 activity is deeply influenced by the membrane viscosity as could be demonstrated performing determinations of the minimal inhibitory concentrations (MIC) at different temperatures and analyzing the membrane viscosity in these cells as well as in resistant bacteria. In all the cases, bacteriocin activit...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2004.10.013
更新日期:2005-02-01 00:00:00
abstract::It is now over 10 years since the discovery of peroxisome proliferator activated receptor gamma (PPAR gamma) and its unique role in adipogenesis. The subsequent identification of PPAR gamma as the target of insulin sensitizing drugs certified this ligand-regulated transcription factor as an exciting link between adipo...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2004.10.021
更新日期:2005-01-01 00:00:00
abstract::Conjugated linoleic acids (CLA) are positional and geometric dienoic isomers of linoleic acid. Dietary CLA supplementation leads to a drop in fat mass in various species, including in humans. The t10,c12-CLA isomer is responsible for this anti-obesity effect. The reduction of fat mass is especially dramatic in the mou...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2004.11.006
更新日期:2005-01-01 00:00:00
abstract::Well-defined quantities of *OH, O2*-,HO2* or RO2*)radicals (reactive oxygen species) can be specifically produced by radiolysis of water or ethanol. Such radical species can initiate one-electron oxidation or one-electron reduction reactions on numerous biological systems. The oxidative hypothesis of atherosclerosis c...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2004.10.007
更新日期:2004-12-01 00:00:00
abstract::Single cell oils (SCOs) are now produced by various microorganisms as commercial sources of arachidonic acid (ARA) and docosahexaenoic acid (DHA). These oils are now used extensively as dietary supplements in infant formulas. An understanding of the underlying biochemistry and genetics of oil accumulation in such micr...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2004.09.017
更新日期:2004-11-01 00:00:00
abstract::Insects have developed an efficient host defense against microorganisms, which involves humoral and cellular mechanisms. Numerous data highlight similarities between defense responses of insects and innate immunity of mammals. The fruit fly, Drosophila melanogaster, is a favorable model system for the analysis of the ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2004.07.007
更新日期:2004-09-01 00:00:00
abstract::New strains of basidiomycetes producing extracellular laccases (Trametes ochracea 92-78, and Trametes hirsuta 56) have been found by screening of isolates of Trametes fungi. The laccases from T. hirsuta 56 and T. ochracea 92-78 as well as two laccases from previously found and described strains of basidiomycetes, name...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2004.08.005
更新日期:2004-09-01 00:00:00
abstract::Hyper- (450 mOsm/l) and hypoosmotic exposure (150 mOsm/l) of Caco-2 cells, a human intestinal epithelial cell line, induced a twofold- and a fivefold increase in the production of IL-8, a constitutively expressed cytokine, respectively. This was observed both in the presence or in the absence of added proinflammatory ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2004.07.009
更新日期:2004-08-01 00:00:00
abstract::Leptin is a cytokine secreted by the adipose tissue that is involved in the control of body weight. We previously showed that a point mutation (R105W) in leptin results in leptin deficiency, marked obesity and hypogonadism in humans adults. Expression in COS1 cells showed impaired secretion and intracellular accumulat...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2004.06.005
更新日期:2004-06-01 00:00:00
abstract::Glycosylphosphatidylinositol phospholipase D (GPI-PLD) has been proposed to be responsible for cleaving membrane-associated glycosylphosphatidyl inositol (GPI) molecules to generate inositol phosphoglycan (IPGs), which have growth factor-mimetic properties. We have cloned the mouse liver GPI-PLD cDNA, which has a sequ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2004.04.003
更新日期:2004-04-01 00:00:00
abstract::The crystallographic structure of a novel trypsin inhibitor (CTI) from Copaifera langsdorffii is reported. The structure was solved by MIRAS procedure and refined to a crystallographic residual of 17.3% (R(free) = 20.3%) at 1.8 A resolution. Two isomorphous derivatives were obtained by quick cryo-soaking approach. CTI...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2004.03.004
更新日期:2004-03-01 00:00:00
abstract::When liver cells from either normal or hereditarily diabetic rats are exposed to (13)C-enriched D-fructose (10 mM) and unlabelled D-glucose (also 10 mM) in the presence of D(2)O, the output of (13)C-enriched D-glucose generated from D-[1-(13)C]fructose is significantly lower than that from D-[2-(13)C]fructose. This co...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2003.12.005
更新日期:2004-02-01 00:00:00
abstract::Anaerobiospirillum succiniciproducens phosphoenolpyruvate (PEP) carboxykinase catalyses the reversible metal-dependent formation of oxaloacetate (OAA) and ATP from PEP, ADP and CO(2). Mutations of PEP carboxykinase have been constructed where the residues His(225) and Asp(263), two residues of the enzyme's putative Mn...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2003.10.013
更新日期:2004-01-01 00:00:00
abstract::DNA mismatch repair (MMR) is an important replication error avoidance mechanism that prevents mutation. The association of defective MMR with familial and sporadic gastrointestinal and endometrial cancer has been acknowledged for some years. More recently, it has become apparent that MMR defects are common in acute my...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2003.10.007
更新日期:2003-11-01 00:00:00
abstract::Rat kidney acylase I was characterised by performing site-directed mutagenesis and enzymatic analysis in the presence of various chemical inhibitors. Site-directed mutagenesis on E147 and overexpression of the protein in a bacterial system, revealed the importance of this residue in enzymatic activity, it corresponds ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2003.09.002
更新日期:2003-10-01 00:00:00
abstract::Since discovery of high-temperature superconductive (HTS) ceramics by Bednorz and Muller in 1986, there has been an accelerated development of cold technologies in industry, including the domain of NMR detection. The purpose of this paper is to fix ideas about the stage that cryogenic radio frequency (RF) probe techni...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2003.09.016
更新日期:2003-09-01 00:00:00
abstract::Ligation of CD47 by its natural ligand thrombospondin (TSP), or cross-linking by CD47 antibodies, triggers caspase-independent cell death in normal and leukemic cells. This kind of cell death is characterised by the cytoplasmic events of apoptosis including externalisation of phosphatidylserines and mitochondria swell...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(03)00129-9
更新日期:2003-08-01 00:00:00
abstract::Three Plasmodium falciparum serine repeat antigen (SERA) protein peptides were studied by NMR and structure calculations being done in 70:30 water:trifluoroethanol solution. Peptide 22834 was shown to be immunogenic and protective against malaria in Aotus monkeys, whilst native peptide 6737 and its analogue 14096 did ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(03)00136-6
更新日期:2003-07-01 00:00:00
abstract::Our principle hypothesis is that the hypothalamic hormone, gonadotropin-releasing hormone (GnRH), is an immunostimulatory hormone and plays a pivotal role in the gender differences in immunity and/or autoimmunity. As a general rule, females display heightened immune responses and heightened endocrinological responsive...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(03)00106-8
更新日期:2003-06-01 00:00:00
abstract::Specific contacts between DNA phosphate groups and positively charged nucleophilic amino acids from the Escherichia coli Fpg protein play a significant role in DNA-Fpg protein interaction. In order to identify these phosphate groups the chemical crosslinking procedure was carried out. The probing of the Fpg protein ac...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(03)00067-1
更新日期:2003-05-01 00:00:00